Vibration can unscrew it, especially if it was not tight in the first place. It can unscrew from vibration is the fuel line is loos on the barbs also, which could also cause an air leak and run problems. The complete fuel system has to be 100% leak proof from the tank to the spray bar. Sucking air, which the engine vacuum can do, anywhere along the way can cause a funny run.
Type at you later,
Dan McEntee